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Thyroid Disorders: Laboratory Diagnosis

The thyroid gland regulates metabolism, energy balance, cardiovascular function, and nervous system activity. Thyroid dysfunction is one of the most common endocrine disorders.

Key Biomarkers

BiomarkerWhat It ShowsNormal Range
TSHPituitary–thyroid axis0.4–4.0 mIU/L
Free T4Active thyroid hormone10–23 pmol/L
Free T3Most active form3.1–6.8 pmol/L

Hypothyroidism (Underactive Thyroid)

Lab pattern: TSH ↑, Free T4 ↓ (or normal in subclinical form)

Symptoms: fatigue, weight gain, cold intolerance, dry skin, constipation, bradycardia

Common causes: Hashimoto's thyroiditis, post-surgical, medication-induced

Hyperthyroidism (Overactive Thyroid)

Lab pattern: TSH ↓, Free T4 ↑ and/or Free T3 ↑

Symptoms: tachycardia, weight loss, tremor, sweating, irritability

Common causes: Graves' disease, toxic adenoma, thyroiditis

Testing Frequency

SituationRecommendation
Screening (no symptoms)Every 5 years after age 35
Levothyroxine dose adjustmentEvery 6–8 weeks
Stable hypothyroidismEvery 6–12 months
PregnancyEvery trimester

FAQ

Is TSH alone sufficient?

For screening — yes. If TSH is abnormal, Free T4 and T3 should be tested to clarify the diagnosis.

Does food affect results?

TSH is best drawn fasting in the morning — levels have a circadian rhythm and may be lower after meals.


Track your thyroid markers with Health Vault — charts help your doctor evaluate treatment effectiveness.

Vert Neo Limited — developer Health Vault